ชื่อเอกสาร

  • Buffalo Bulletin (Thailand)

  • Vol. 37 NO. 1 Page 71-80

  • http://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/907

  • en

  • L73-โรคสัตว์

  • Brucellosis has been one of the most important reproductive diseases among different livestock species as well as animal handlers and is considered a reemerging infectious disease in many areas of the world. A total of 353 clinical samples of cattle (207) and buffalo (146) were collected for molecular detection of Brucella. Out of 353 clinical samples, 6 samples (vaginal discharge-2, placenta-2, foetal stomach content-1, fetal lung-1) positive for Brucella organism by genus specific PCR based on bscp31 (B4/B5 primer, 223bp). All six genus specific positive sample also positive for B. abortus +IS711 (498bp) species specific PCR. All the samples which were detected positive in conventional PCR also detected positive for Brucella in SYBR green and TaqMan probe based real time PCR.

  • Buffalo;Bubalus bubalis;Brucellosis;Molecular;Detection;PCR;Bscp31;B4/B5;IS711 primer;Gene;India

  • [1] Kirit B. Patel (Sardarkrushinagar Dantiwada Agricultural University, Gujarat (India). College of Veterinary Science and Animal Husbandry. Department of Animal Biotechnology and Veterinary Microbiology)
    [2] H.C. Chauhan (Sardarkrushinagar Dantiwada Agricultural University, Gujarat (India). College of Veterinary Science and Animal Husbandry. Department of Animal Biotechnology and Veterinary Microbiology)
    [3] S.S. Patel (Sardarkrushinagar Dantiwada Agricultural University, Gujarat (India). College of Veterinary Science and Animal Husbandry. Department of Animal Biotechnology and Veterinary Microbiology)
    [4] B.K. Patel (Sardarkrushinagar Dantiwada Agricultural University, Gujarat (India). College of Veterinary Science and Animal Husbandry. Department of Animal Biotechnology and Veterinary Microbiology)
    [5] M.D. Shrimali (Sardarkrushinagar Dantiwada Agricultural University, Gujarat (India). College of Veterinary Science and Animal Husbandry. Department of Animal Biotechnology and Veterinary Microbiology)
    [6] B.S. Chandel (Sardarkrushinagar Dantiwada Agricultural University, Gujarat (India). College of Veterinary Science and Animal Husbandry. Department of Animal Biotechnology and Veterinary Microbiology)

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Kirit B. Patel and others. (2018). Molecular detection of Brucella abortus using bscp31 and IS711 gene based pcr assay in cattle and buffalo.  Buffalo Bulletin (Thailand), 37 (1) ,71-80

Kirit B. Patel and others. "Molecular detection of Brucella abortus using bscp31 and IS711 gene based pcr assay in cattle and buffalo" Buffalo Bulletin (Thailand), 37, 2018, 71-80.

Kirit B. Patel and others. "Molecular detection of Brucella abortus using bscp31 and IS711 gene based pcr assay in cattle and buffalo" Buffalo Bulletin (Thailand) .37.1 (2018):71-80.print.